High-affinity choline uptake protein bett

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August undefined, 2024

Web5 de mai. de 2024 · The high-affinity choline transporter CHT1 mediates choline uptake, the rate-limiting and regulatory step in acetylcholine synthesis at cholinergic presynaptic … WebIn cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the …

Choline Uptake - an overview ScienceDirect Topics

WebE. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA … WebThe nature of the high affinity uptake system guarantees that cholinergic cells will always receive priority access to all available choline. Because of this, choline will be … c# sort array ascending

High-affinity choline transporter - PubMed

Web8 de mar. de 2006 · The high-affinity choline transporter at the molecular level. Despite the fact that the high-affinity choline uptake system was described in the 1960s, the genes … WebA molecular phylogenetic analysis of salt-tolerance-related genes was carried out using complete genome sequencing data available for 26 Sinorhizobium meliloti strains and for 25 bacterial strains belonging to 17 genera. It was revealed that the genes of the first and the second stages of the response to salt stress (aqpZ, trkH, and trkA, and betICBA) have … WebCholine uptake High affinity Na+-dependent Synaptosomes Certain basic characteristics of choline uptake in nerve terminals were ... protein, 0,567 uM [3H]-choline (final concentration), for 20 min at 371. The KM value of [3H]-choline uptake was 2.27+0.26 10 20 30 40 Time (min) 60 B I c# sort array descending

Pseudomonas syringae BetT Is a Low-Afﬁnity Choline

Competitive inhibition of the high-affinity choline transporter by ...

WebThis transporter has a particularly low affinity (K(m) of 876 microM) and high capacity (V(max) of 80 nmol/min/mg of protein) for choline transport relative to other known … Web1 de jan. de 2024 · CHT1 has been functionally characterized by its capacity to transport choline with high affinity when expressed in cultured cells or Xenopus oocytes. CHT1 transports choline with a Km value of 1–5 μM in a Na + - and Cl −-dependent manner (Okuda et al. 2000).The only known specific inhibitor of CHT1 is hemicholinium-3 (HC-3), … ealing bowel and bladder serviceWeb28 de mar. de 2003 · Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Here, we investigated by RT … ealing boulevard

"Web29 de mar. de 2024 · This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from … " - High-affinity choline uptake protein bett

High-affinity choline uptake protein bett

Web29 de jul. de 2014 · The high-affinity choline uptake activity was also shown to depend on the kind of anions and on the concentrations of K +: the highest activity was observed in the presence of Cl − and 0.35–4.8 mM K +, together with 126 mM Na + . Selective distribution and characterization of the high-affinity choline uptake system in cholinergic neurons Web1 de jan. de 2024 · The high-affinity choline uptake activity in patients with these diseases was predicted to be diminished by mutations that impaired CHT1 function and/or trafficking. There is a functionally relevant, nonsynonymous single nucleotide polymorphism in the coding region of the human CHT1 gene (c.265A>G, rs1013940) (Okuda et al. 2002 ).

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Web1 de abr. de 2024 · Furthermore, the genome contains nine genes related to the synthesis and transport of the compatible-solute glycine betaine for resistance to osmotic stress … Web8 de nov. de 2005 · The choline uptake activity is completely inhibited by the protonophore carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP). 1 publication …

Web8 de mar. de 2006 · The high-affinity choline transporter at the molecular level. Despite the fact that the high-affinity choline uptake system was described in the 1960s, the genes for the high-affinity choline transporter proteins (CHT1) of Caenorhabditis elegans and rat have only recently been cloned (Okuda et al. 2000). WebHigh affinity choline uptake is generally believed to be the rate-limiting step in acetylcholine synthesis in cholinergic neurons and is essential for cholinergic ... hCHT1 protein is composed of 580 amino acids with a predicted molecular mass of 63 kDa. Sequence analysis of the hCHT1 protein predicts 13 transmembrane domains (13, 20, 21).

WebProtein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. … WebCholine uptake (Vmax) increases up to 3 months of age and decreases significantly from 1 to 7 years of age ( Fig. 6A; Table III ). A further indication of a reduced synthesis with …

WebMeyer EM, Cooper JR (1983) High affinity choline uptake and calcium-dependent acetylcholine release in proteoliposomes derived from rat cortical synaptosomes. J …

WebProtein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. Proteomes. Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) ealing borough demographicsWebA choline uptake system with a high affinity for choline has long been assumed to be present in cholinergic neurons. Very recently, the molecular entity for the high-affinity … ealing borough populationWeb23 de dez. de 2009 · We identified a choline, betaine and carnitine transporter, designated Cbc, from Pseudomonas syringae and Pseudomonas aeruginosa that is unusual among members of the ATP-binding cassette (ABC) transporter family in its use of multiple periplasmic substrate-binding proteins (SBPs) that are highly specific for their … ealing bottomless brunchWebProtein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. … ealing borough mapWebThe presumed binding proteins of both ABC transporters were shown to be lipoproteins. ... -choline uptake via OpuB (K(m) = 1 microM; Vmax = 21 nmol min-1 mg-1 protein) and OpuC (K(m) = 38 microM; Vmax = 75 nmol min-1 mg-1 protein) revealed that each of these ABC transporters exhibits high affinity and substantial transport capacity. c# sort array exampleWebGene ID: 13909447, discontinued on 16-Jul-2024. Summary Other designations. High-affinity choline uptake protein BetT c# sort array in ascending orderWebProtein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. Proteomes. Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) ealing boundary map